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Declaration
1. There have been incidents of fraudulent individuals using fake websites to deceive authors into submitting manuscripts, as well as contacting authors under the guise of our editorial office and its staff. This has caused significant harm to our journal's reputation. Authors are strongly urged to remain vigilant. We have reported these activities to the police and reserve the right to pursue legal action against such individuals. We would like to emphasize that our journal does not collaborate with any agencies or individuals to publish articles for a fee. We do not accept email submissions. The official website of our editorial office is: https://qkzx.henau.edu.cn/, and the manuscript submission site is https://nnxb.cbpt.cnki.net/. Please be aware to avoid being deceived.
2. Recently, some fraudsters have been impersonating our editorial office and sending emails to authors by sending letters to authors through email addresses such as qilinshou0713@163.com and 13643188379@163.com to extort various fees under various names such as random review of publication quality and download of proofs. Please note that the correct email address for our journal is hnndxb@henau.edu.cn.
3. Our journal currently does not charge any review fees. However, we do charge a publication fee, which is required to be paid within one week of the receipt of relevant notification. If payment is not made on time, the publication of the article may be delayed or canceled. Authors not affiliated with Henan Agricultural University should pay via bank transfer, while internal transfers should be used by authors from our university.
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Account Name: Henan Agricultural University
Account Number: 1600 3101 0400 06945
Bank: Agricultural Bank of China, Zhengzhou Shangdu Branch
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Please include the note "Author's Name + Publication Fee for Journal of Henan Agricultural University" when making the transfer, and promptly notify us of the transfer time via email to facilitate account verification.
5. Our journal has never collaborated with any agencies. Please do not fall victim to scams.
6. Once a manuscript is accepted, it will be eligible for online first publication. However, authors must ensure that the title, authorship and order, affiliations, and other information remain unchanged. Any issues or liabilities arising from changes after online first publication will be the responsibility of the authors.
Journal Introduction
Journal of Henan Agricultural University is committed to the principles of rigor, pragmatism, innovation and growth. It aims to promote agricultural science and technology and contribute to the development of related disciplines. The journal participates in the progress of the university, academic disciplines, scientific growth and achievements of researchers. The journal primarily publishes articles in the fields of crop science, veterinary medicine, agricultural engineering, forestry, animal husbandry, agricultural and forestry economics and management, biology, agricultural resources and environment, food science and engineering, landscape architecture, ecology, plant protection, horticulture, and tobacco science. Manuscripts are expected to demonstrate scientific rigor, originality, authenticity, and adherence to academic standards.
The journal is currently included in many international databases, including the Chemical Abstracts (US), Japan Science & Technology Agency Database, databases of CABI (UK), EuroPub and EBSCO. It has been indexed in A Guide to the Core Journals of China (2023 edition) and selected as a Key Source Journal for Chinese Science Citation Database (CSCD) (2025-2026), a Key Magazine of China Technology (Source Journal for Chinese Scientific and Technical Papers and Citations), a China Core Academic Journal by Research Center for Chinese Science Evaluation (RCCSE), a Core Journal of Agriculture and Forestry in China (Class A), and a High-quality Scientific Journal in the Field of Agriculture and Forestry by China Association for Science and Technology. In addition, the journal has received numerous awards, including National Excellent Scientific Journal, Double-Effect Journal of China Scientific Journal Phalanx, Outstanding Natural Science Journal of Chinese Higher Education Institutions, Excellent Scientific Journal of Chinese Universities, Demonstration Case of Excellent Scientific Journal Construction in Chinese Universities, Premium Journal of Chinese Agricultural Journals, Outstanding Team of Chinese Agricultural Journals, Most Influential Agricultural Journal in China, Henan Excellent Publication Award, Outstanding Scientific Journal of Henan Province, Excellent University Journal of Henan Province, and First-Class Journal of Henan Province.
Research progress on different types of mucosal adjuvants and their application in veterinary vaccines
YANG Haoxiang;ZHOU Hengqi;LIU Ziyang;DUAN Laitong;HE Hua;This review summarizes the major types and research progress in the application of respiratory mucosal immune adjuvants, with particular emphasis on the characteristics and underlying mechanisms of microbial-derived mucosal adjuvants, pattern recognition receptor agonist-based mucosal adjuvants, natural product-derived mucosal adjuvants, and synthetic nanomaterial-based mucosal adjuvants in enhancing mucosal barrier immunity, inducing secretory IgA, and promoting cellular immune responses.It also summarizes their application progress in veterinary vaccines against porcine reproductive and respiratory syndrome, avian influenza, and infectious bronchitis in chickens.Currently, studies on veterinary respiratory mucosal adjuvants still lack systematic data on safety, dose optimization, and challenge protection in target animals, such as pigs and poultry.Future research should integrate immunology and materials engineering to advance target-animal immunoregulation and scenario-oriented validation, thereby providing a reference for the design of veterinary mucosal vaccine adjuvants.
Research progress on the application of synthetic microbial communities in plant regulation
YAO Yicai;ZHANG Yuqi;YU Kejia;ZHOU Yikai;ZHOU Wang;WU Jiaxuan;SHI Mingzi;WANG Mingdao;This paper summarizes the characteristics and construction strategies of synthetic microbial communities (“top-down” “bottom-up” and “integrated approaches”), and reviews their functions(promoting plant growth, controlling diseases, and resisting abiotic stress) and mechanisms of action(stable root colonization, metabolic flux balance, and induction of stress resistance) in plant regulation.Currently, synthetic microbial communities still face challenges such as complex structures, difficult colonization, and production difficulties.Future research needs to deeply integrate multi-omics, artificial intelligence, and synthetic biology tools, to construct multi-trophic microbial communities through ecological network analysis and machine learning, and to promote the transition of synthetic microbial communities from “empirical combinations” to “rational design”.
Cloning and functional analysis of the maize transcription factor ZmbHLH54 in response to drought stress
YANG Ziqiong;SONG Bolin;ZHANG Nannan;MENG Jiaxing;HE Xueyuan;FAN Xiaowei;WU Jianyu;【Objective】To identify a novel drought-responsive gene, ZmbHLH54, belonging to the basic Helix-Loop-Helix(bHLH)transcription factor family, and to investigate the response mechanism of this gene in a drought-stressed environment.【Method】Bioinformatics approaches were employed to systematically analyze the gene structure, protein characteristics, phylogenetic relationships, conserved motifs, and promoter cis-acting elements.Furthermore, the phenotypic identification of gene mutant material was conducted under drought stress conditions.The activities of superoxide dismutase(SOD) and peroxidase(POD) were measured, and the expression levels of relevant genes were quantified using real-time fluorescent quantitative PCR(RT-qPCR).These analyses provided preliminary insights into the mechanisms underlying drought resistance.【Result】The ZmbHLH54 gene has a typical bHLH domain and belongs to the bHLH transcription factor family.The coding region is 690 bp, encoding 229 amino acids.There are several elements related to hormone and drought stress response in promoter region.ZmbHLH54 is a hydrophilic protein without transmembrane helical structure.After 5 days of drought stress, the negative control plants showed obvious leaf curling and wilting, while most mutant seedlings still kept the leaf stretch state or only slightly curling.Under drought stress, SOD and POD activities in zmbhlh54 mutant were significantly higher than those in the negative control.Furthermore, the expression of reactive oxygen species(ROS) scavenging genes(ZmSOD3 and ZmSOD4) was up-regulated in zmbhlh54 mutant, while the expression of ROS-related gene(Zm00001d036557) was suppressed.【Conclusion】The bHLH transcription factor family ZmbHLH54 may mainly respond to drought stress by regulating ROS clearance mechanism under drought stress, and enhances drought resistance of mutant at seedling stage.
Effects of different plant growth regulators on walnut fruit quality
ZHANG Tao;CHEN Xiaolong;QIAO Xinpai;DING Shen;YANG Chaochen;LAI Yong;ZHANG Dangquan;【Objective】To investigate the effects of plant growth regulators on the drupe weight, kernel weight and fatty acid content in walnut fruit, providing a scientific basis for improving the quality of walnut.【Method】In this study, walnut was treated with 6-BA(50 mg·L-1), salicylic acid(SA, 100 mg·L-1) and paclobutrazol(PAC, 50 mg·L-1) to appraisal the fruit phenotype and analyze the changes of oil content and fatty acid composition.【Result】After 6-BA treatment, the drupe weight was significantly increased by 21.84%(p<0.01), 5.96% and 3.92%(p<0.05) on the 1 st, 7 th and 14 th day, respectively, while the oil content was decreased by 16.29% and 3.44%(p<0.01) on the 1 st and 28 th day, respectively.The relative content of linoleic acid was increased, leading to enhanced the accumulation of polyunsaturated fatty acids under 6-BA treatment.After SA treatment, the drupe weight was significantly decreased by 6.31%, 5.17%(p<0.05) and 7.23%(p<0.01) on the 14 th, maturity and 28 th day, respectively, and the kernel weight was significantly decreased by 8.09%, 4.35%(p<0.05) and 8.44%(p<0.01) on the 7 th, maturity and 28 th day.The oil content was significantly decreased by 14.79%(p<0.01) on the 1 st day but increased by 3.52%(p<0.05) on the 14 th day.SA treatment inhibited the accumulation of unsaturated fatty acids and polyunsaturated fatty acids, and showed a pattern of initial inhibition followed by promotion in oleic acid.It also inhibited the accumulation of linoleic acid and linolenic acid.After PAC treatment, the kernel weight was significantly increased by 5.83%, 6.61% and 4.11%(p<0.05) on the 1 st, 14 th and 28 th day.The oil content was increased by 2.45%, 1.21%(p<0.05) and 6.60%(p<0.01) on the 1 st, 7 th and 14 th day.The action on polyunsaturated fatty acids was firstly promotive and then inhibitory.
Effects of harvest time on bacterial community structure and quality of cigar tobacco leaves during air-curing
PEI Yubing;WANG Zhenhua;TAN Yonghao;LIU Jie;QUAN Yihua;WANG Wei;SHI Xiangdong;DING Songshuang;Key Laboratory of Tobacco Cultivation in Tobacco Industry of Henan Agricultural University;【Objective】The impact of harvesting time on the bacterial community structure and quality of cigar tobacco leaves during air-curing was investigated to offer a theoretical foundation and technical guidance for the production of high-quality domestic cigar tobacco leaves.【Method】 The Cuba-7 variety was used as the experimental material in this study, and three harvest time treatments were set up(T1: harvested at 20 days after topping; T2: harvested at 25 days after topping; T3: harvested at 30 days after topping).The changes in bacterial community structure, appearance quality, main carbon and nitrogen compounds content and sensory quality of cigar tobacco leaves harvested at different times during air-curing were analyzed.【Result】With the delay of harvest time, the species of bacterial community on the surface of tobacco leaves increased, but the dominant bacteria on the surface of tobacco leaves in different treatments were the same, all of which were Sphingomonas.During the air-curing process, the bacterial genera on the surface of tobacco leaves exhibited predominantly positive correlations, indicating a strong symbiotic relationship.The later the tobacco leaves were harvested, the faster the color changed during the air-curing process, especially during the yellowing and browning periods.The content of carbon-containing compounds in fresh tobacco leaves was T3 > T2 > T1, while the content of nitrogen-containing compounds showed the opposite trend.Within a specific limit, the smoke and style attributes of tobacco leaves intensify as the harvesting time is postponed.【Conclusion】The dominant genus of cigar tobacco leaves with different harvest time in the air-curing process was the same, all of which were Sphingomonas.Appropriately delaying harvest can increase the species richness of the dominant genus on the surface of fresh tobacco leaves, and notably reduces the protein content in fresh tobacco leaves, which in turn boosts the smoke concentration and permeability of the tobacco leaves following fermentation.Meanwhile, the aroma of woody, sweet and pollen can be highlighted, and the quality can be significantly improved.
Journal Information

Journal of Henan Agricultural University
(Bimonthly, founded in 1960)
Governing Body: Henan Agricultural University
Sponsor: Henan Agricultural University
Publisher: Editorial Office of Journal of Henan Agricultural University
(No. 218, Ping'an Avenue, Zhengdong New District, Zhengzhou, China)
Postal Code: 450046
Tel: +86-371-56552551
E-mail: hnndxb@henau.edu.cn
Editor-in-Chief: ZHOU Wei
Subscription Office: All Local Post Offices in China
Postal Distribution Code: 36-132
Domestic Distribution: Henan Provincial Newspaper and Publication DistributionBureau of China Post Croup Co., Ltd.
International Distribution: China International Book TradingCorporation (P.O. Box399, Beijing, China)
International Distribution Code: BM5953
ISSN: 1000-2340
CN: 41-1112/S
WeChat Official Account: hnndxb1985
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