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【Objective】To identify cyclin(CYCs) gene family members across the whole genome of Paulownia fortunei(PF) and investigate their roles in the occurrence of paulownia witches' broom disease.【Method】Bioinformatics methods were employed to identify PfCYCs family members from the whole genome of Paulownia fortune, and to analyze their phylogenetic relationships, gene structures, physicochemical properties, and collinearity.The promoter cis-acting element features and proteinprotein interactions(PPI) were also predicted.Based on transcriptome sequencing data from Paulownia fortunei and Paulownia fortunei infected with phytoplasmas(PFI), the expression changes of PfCYCs genes in PF and PFI were analyzed.【Result】A total of 48 CYCs family members were identified in Paulownia fortunei, which were unevenly distributed across 20 chromosomes.Phylogenetic analysis classified PfCYCs into six types.Collinearity analysis revealed that 32 PfCYCs were involved in segmental duplication events.PPI analysis indicated that type A and B CYCs interact with proteins such as WEE1, while type D CYCs interact with proteins such as RBR1.Flow cytometry showed that Paulownia witches' broom disease alters the cell cycle and affects cell numbers, particularly in the G2 and S phases.Transcriptome analysis revealed that 22 PfCYCs genes were upregulated and 4 genes were downregulated in PFI.【Conclusion】In the genome of Paulownia fortunei, a total of 48 PfCYCs genes were identified, among which PfCYCD3;1 and PfCYCD5;1 are likely closely associated with the occurrence of witches' broom disease.
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Basic Information:
DOI：10.16445/j.cnki.1000-2340.20250407.004
China Classification Code:S792.43
Citation Information:
[1]WU Wenhao,LI Yujie,FAN Jiaming ,et al.Analysis of the cyclin gene family in Paulownia fortunei and its expression in response to phytoplasma infection[J].Journal of Henan Agricultural University,2026,60(02):237-250.DOI:10.16445/j.cnki.1000-2340.20250407.004.
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