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【Objective】To screen salt-alkali tolerant plant growth-promoting rhizobacteria(PGPR) with growth-promoting capabilities, so as to provide a theoretical basis and bacterial resources for the improvement of saline-alkali soils and the development of microbial fertilizers.【Method】Rhizosphere soil samples of peanut plants were collected from Hami, Xinjiang Uygur Autonomous Region, and bac‑teria were isolated and screened using Luria-Bertani(LB) medium.Based on morphological characteristics, physiological and biochemical properties, and 16S rRNA gene sequence analysis, the target strain was identified.The growth capacity of the target strain was evaluated under varying NaCl concen‑trations(0-200 g·L-1), temperatures(5-55 ℃), and pH levels(4-13) to determine its salt tolerance range, temperature tolerance, and pH tolerance.The multiple functional identification media were used to assess the plant growth-promoting functions, including amylase production, siderophore syn‑thesis, and cellulase production.Additionally, the capacity for amylase production and biofilm forma‑tion was quantitatively determined.Through plate and pot experiments, the growth-promoting effects on tomato and maize seedlings were evaluated, respectively.【Result】A bacterial strain was isolated and identified as Bacillus halotolerans, designated as Bacillus halotolerans ZP-3821.Strain ZP-3821 was capable of growing within a NaCl concentration range of 0-40 g·L-1, a temperature range of 20-50 ℃, and a pH range of 5-10.The strain exhibited the abilities of fixing nitrogen, producing amy‑lase, synthesizing siderophores, secreting cellulase, and forming biofilm.The plate experiment results showed that strain ZP-3821 significantly enhanced the aboveground fresh weight, underground fresh weight, and lateral roots number of tomato seedlings under saline-alkali stress.Under the condi‑tion of pH value 8.0+2.0 mmol·L-1 NaHCO_3+0 mmol·L-1 NaCl, compared with the control group, inoculation with ZP-3821 increased the aboveground fresh weight, underground fresh weight, and lateral roots number by 169.04%, 198.37%, and 509.48%, respectively.Pot experiment results indi‑cated that under salt stress with a NaCl concentration of 6 g·kg-1, compared to the control, inoculation with strain ZP-3821 increased the underground fresh weight and total fresh weight of maize seedlings by 39.82% and 18.65%, respectively.【Conclusion】Bacillus halotolerans ZP-3821 exhibits excellent salt-alkali tolerance and can promote plant growth under salt-stressed conditions.
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Basic Information:
DOI：10.16445/j.cnki.1000-2340.20251125.001
China Classification Code:S154.3
Citation Information:
[1]WANG Mengqiu,DAI Jinping,YANG Xinping ,et al.Isolation and identification of growth-promoting ability of Bacillus halotolerans ZP-3821[J].Journal of Henan Agricultural University,2026,60(03):492-502.DOI:10.16445/j.cnki.1000-2340.20251125.001.
Fund Information:
新疆维吾尔自治区重点研发任务专项（2022B02019）; 南疆重点产业创新发展支撑计划项目（2022DB026）
2025-11-26
2025-11-26
2025-11-26